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Influence of deposits on algae growth in a tubular photobioreactor
Vajaýová, Veronika ; Lošák, Pavel (referee) ; Naď, Martin (advisor)
The content of the theoretical part of this bachelor thesis is a description of the tubular pho-tobioreactors, comparison of the individual types of the cultivation devices, the fundamental parameters influencing the efficiency of biomass production, and problems of formation of deposits on the inner surface of the photobioreactor. The attention is focused on the effect of sediment formation on the growth of microalgae in the tubular photobioreactor. Since the sediments affect the resulting light intensity in the suspension in the tubes, this phenomenon is reflected in the overall cultivation efficiency and the biomass yield. The practical part presents the course of measurements performed in the vertical tubular photobioreactor designed for flue gas cleaning using the microalgae Chlorella pyrenoidos Chick (IPPAS C2). The aim was to solve the problem of foaming, which subsequently caused deposits in the system's tubes or other parts. To this end, two measures were tested: • the mechanical one in the form of a wire mesh, • the chemical one using sunflower oil. The measured values were the basis for determining the effect of deposits on light intensity and also served to evaluate the functionality of the tested measures.
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Numerical modeling of special LED ligth source
Nepor, František ; Fiala, Pavel (referee) ; Steinbauer, Miloslav (advisor)
This work presents information about numeric simulation of special light sources based on LED. In first, theoretical problems of light simulation are analyzed, next the LED source is designed and finally experimental measurement is done to confirm the results. The target is to aim maximal homogeneity due appropriate layout of LEDs. Content of this work is divided into several parts. In first part the measurement units used in light sources description are explained to understand next parts of the document. Second part describes some of the methods used for simulation of the light sources. Next chapter explains procedure for selection of the method and deals with simulation of light source design in MATLAB. Last part of this work is focused on experimental verification of designed light source, when measured data are compared with the simulated results.
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Methods for evaluation of quality of automotive filters
Sedlák, Jiří ; Drexler, Petr (referee) ; Mikulka, Jan (advisor)
This bachelor thesis deals with theoretical research, description and design of three individual methods of defective air filters detection. Individual methods are described and compared in terms of their technical difficulty and expected efficiency. These methods are optical test, acoustic test, pressure test and the method of detection of gas CO2. All researched methods were proved practically and the measurements of detection of defective air filters were taken. The defect of air filter was detected by use of optical test.
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Efficient Computation of Lighting
Kubovčík, Tomáš ; Pečiva, Jan (referee) ; Milet, Tomáš (advisor)
This bachelor's thesis deals with efficient computation of lighting in graphics scenes containing many light sources. Basic lighting calculation techniques as well as techniques derived from them will be introduced. The main goal of this thesis is to investigate tiled shading and its optimalizations described more in detail in both theoretical and practical sections. Concluding part consists of experiments performed on this techniques measuring their efficiency as well as implementation details of some interesting or important parts of them.
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Proteom rostlin v reakci na abiotický stres
Čotková, Veronika
Plants as a sessile organisms have evolved complex mechanisms to cope with unfavorable environmental conditions. This diploma thesis summarizes current state of knowledge and focuses on light, temperature and phytohormones in plant abiotic stress responses. The practical part is devoted to proteomics and completes and extends the project started during my Bachelor program. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S> GR> HvCKX2) were profiled to elucidate proteome-wide responses to down-regulation of cytokinin levels under heat stress at standard (80 umol.m-2.s-1) and low light intensity (20 umol.m-2.s-1). In total, 2-DE analysis and mass spectrometry analysis revealed 63 differentially abundant proteins involved in diverse metabolic processes. These data provide evidence of a link between temperature, light and cytokinin signaling in Arabidopsis and will be used in the modeling of cytokinin-light-temperature interactions.
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Influence of deposits on algae growth in a tubular photobioreactor
Vajaýová, Veronika ; Lošák, Pavel (referee) ; Naď, Martin (advisor)
The content of the theoretical part of this bachelor thesis is a description of the tubular pho-tobioreactors, comparison of the individual types of the cultivation devices, the fundamental parameters influencing the efficiency of biomass production, and problems of formation of deposits on the inner surface of the photobioreactor. The attention is focused on the effect of sediment formation on the growth of microalgae in the tubular photobioreactor. Since the sediments affect the resulting light intensity in the suspension in the tubes, this phenomenon is reflected in the overall cultivation efficiency and the biomass yield. The practical part presents the course of measurements performed in the vertical tubular photobioreactor designed for flue gas cleaning using the microalgae Chlorella pyrenoidos Chick (IPPAS C2). The aim was to solve the problem of foaming, which subsequently caused deposits in the system's tubes or other parts. To this end, two measures were tested: • the mechanical one in the form of a wire mesh, • the chemical one using sunflower oil. The measured values were the basis for determining the effect of deposits on light intensity and also served to evaluate the functionality of the tested measures.
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Light and its effect on plant proteome
Luklová, Markéta
Light is one of the main environmental factors largely determining plant life. Light provides energy resources for photosynthesis and it is also the most important medium of perception as it represents the main source of information about plant surroundings. My diploma thesis entitled “Light and its effect on plant proteome” briefly summarizes present knowledge about the light perception in plants, its interpretation of light quality, quantity, and direction via photoreceptors. These components trigger a whole range of changes, both on molecular and developmental levels, but the complex molecular mechanisms regulating system of light signalling in plants are far from being resolved. Here, I followed effects of different light parameters on plant proteome, namely, diurnal variations, low- and high-light intensity, spectral composition, and a change in the light period duration. To study these effects two model organisms were selected (i) Arabidopsis thaliana as a representative plant model with the most annotated genome/proteome and (ii) Solanum lycopersicum as a representative plant model significant for agriculture and biotechnology. Proteome changes in response to different light factors were analyzed by LC-MS. In total, the analyses revealed over 100 and 300 light-responsive proteins in Arabidopsis and S. lycopersicum, respectively. Proteomics provided highly sensitive snapshots of protein abundance in response to light and selected experiments have been complemented with a GC-MS metabolite profiling, conductivity measurements, and fluorescence microscopy.
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Proteom rostlin v reakci na abiotický stres
Čotková, Veronika
Plants as a sessile organisms have evolved complex mechanisms to cope with unfavorable environmental conditions. This diploma thesis summarizes current state of knowledge and focuses on light, temperature and phytohormones in plant abiotic stress responses. The practical part is devoted to proteomics and completes and extends the project started during my Bachelor program. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S> GR> HvCKX2) were profiled to elucidate proteome-wide responses to down-regulation of cytokinin levels under heat stress at standard (80 umol.m-2.s-1) and low light intensity (20 umol.m-2.s-1). In total, 2-DE analysis and mass spectrometry analysis revealed 63 differentially abundant proteins involved in diverse metabolic processes. These data provide evidence of a link between temperature, light and cytokinin signaling in Arabidopsis and will be used in the modeling of cytokinin-light-temperature interactions.
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